The Recombinant Mouse VEGF-A/VEGF164 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate VEGF-A/VEGF164 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB0810) is produced using HEK293 cells and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 20.12 kDa with an observed molecular weight of 25-30 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse VEGF-A/VEGF164 Protein (RPCB0810), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse VEGF164 at 1 μg/mL (100 μL/well) can bind Recombinant Human VEGFR2 with a linear range of 8-30 ng/mL.
Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED 50 for this effect is typically 0.006-0.022 ng/mL, corresponding to a specific activity of 4.54×10 7 -1.67×10 8 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Mouse VEGF-A/VEGF164 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Recombinant Mouse VEGF164 at 1 μg/mL (100 μL/well) can bind Recombinant Human VEGFR2 with a linear range of 8-30 ng/mL.
