Description
Recombinant Rat c-Kit ligand/KITLG/SCF Protein
The Recombinant Rat c-Kit ligand/KITLG/SCF Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB1811) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 19.17 kDa with an observed molecular weight of 25-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Rat c-Kit ligand/KITLG/SCF Protein |
| SKU: | RPCB1811 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Rat |
| Synonyms: | Mgf, SCF, Kitl, c-Kit ligand |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 19.17 kDa |
| Observed MW: | 25-40 kDa |
| Gene ID: | 60427 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Rat c-Kit ligand/KITLG/SCF Protein (RPCB1811), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.01 EU/μg of the protein by LAL method |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Measured in a cell proliferation assay using MC/9‑2 mouse mast cells.The ED50 for this effect is 9.95-39.78 ng/mL, corresponding to a specific activity of 2.51×10 4 ~ 1.01× 10 5 units/mg. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Similar to Kit ligand precursor (C-kit ligand), also known as Stem cell factor (SCF), Mast cell growth factor (MGF), or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein can augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.
 | Recombinant Rat c-Kit ligand/KITLG/SCF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Recombinant Rat c-Kit ligand/SCF/KITLG stimulates cell proliferation of the MC/9‑2 mouse mast cells.The ED50 for this effect is 9.95-39.78 ng/mL, corresponding to a specific activity of 2.51×104~ 1.01× 10 5 units/mg. |
