Description
Recombinant Rat IL-4 Protein
The Recombinant Rat IL-4 Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB2008) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 14.60 kDa with an observed molecular weight of 15-25 kDa under denaturing conditions, achieving ≥ 90% as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Rat IL-4 Protein |
| SKU: | RPCB2008 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Rat |
| Synonyms: | B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1, H-IL-4, IL-4, ILN, Interleukin, Interleukin-4, Interleukin4, Lymphocyte stimulatory factor 1, M-IL-4, MGC79402 |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 14.60 kDa |
| Observed MW: | 15-25 kDa |
| Gene ID: | 287287 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Rat IL-4 Protein (RPCB2008), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.01 EU/μg of the protein by LAL method |
| Purity: | ≥ 90% as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED 50 for this effect is 0.65-2.6 μg/mL, corresponding to a specific activity of 3.85×10 2 ~1.54×10 3 units/mg. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
 | Recombinant Rat IL-4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Recombinant Rat IL-4 stimulates cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.65-2.6 μg/mL, corresponding to a specific activity of 3.85×102~1.54×103 units/mg. |
