Description
Recombinant Rat IL-9 Protein
The Recombinant Rat IL-9 Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB1718) is produced using HEK293 cells and features a C-6His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 14.98 kDa with an observed molecular weight of 30-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography | |
Mammalian & Bacterial Expression Systems | |
High lot-to-lot consistency via strict QC |
Product Name: | Recombinant Rat IL-9 Protein |
SKU: | RPCB1718 |
Size: | 10 μg , 20 μg , 50 μg , 100 μg |
Reactivity: | Rat |
Synonyms: | IL9 |
Tag: | C-6His |
Expression Host: | HEK293 cells |
Calculated MW: | 14.98 kDa |
Observed MW: | 30-40 kDa |
Gene ID: | 116558 |
Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Rat IL-9 Protein (RPCB1718), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
Purity: | ≥ 95 % as determined by SDS-PAGE. |
Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
Bio-Activity: | Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED 50 for this effect is 8.02-32.1 ng/mL. |
Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
IL-9 is a pleiotropic cytokine that influences various distinct functions of different target cells such as T cells, B cells, mast cells and airway epithelial cells by activating STAT1, STAT3 and STAT5. Because of its pleiotropic functions, IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases.