Sclerostin, the protein product of the SOST gene, is a potent inhibitor of bone formation. Sclerostin protein is widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days, and was originally identified as an important regulator of bone remodeling, homeostasis, and links bone resorption and bone apposition. Recent studies have revealed that Sclerostin protein inhibits the bone growth probably by binding to the extracellular domain of the Wnt coreceptors LRP5 and LRP6 and disrupting Wnt-induced Frizzled-LRP complex formation.
Product Name:
Rat Sclerostin/SOST Recombinant Protein (RPES1519)
Product Code:
RPES1519
Size:
10µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
SOST
Accession:
NP_085073.1
Sequence:
Met 1-Tyr 213
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat SOST (NP_085073.1) (Met 1-Tyr 213) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
23 kDa
AP Mol Mass:
33 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
