Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein
The Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB2051) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 26.2 kDa with an observed molecular weight of 30-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein
SKU:
RPCB2051
Size:
100 μg
Reactivity:
SARS-COV-2
Synonyms:
S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
High quality, high purity and low endotoxin recombinant Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein (RPCB2051), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Immobilized SARS-CoV-2 Spike RBD (N501Y,K417N,E484K) at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC 50 of 0.48 μg/mL determined by ELISA.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of SARS-COV-2 Spike RBD (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.
