Recombinant SARS-CoV-2 Spike S1+S2 ECD (S-ECD) (B.1.1.529/Omicron) Trimer Protein
The Recombinant SARS-CoV-2 Spike S1+S2 ECD (S-ECD) (B.1.1.529/Omicron) Trimer Protein represents a critical molecular component of the SARS-CoV-2 virus, playing an essential role in viral entry and host cell infection mechanisms. This protein is extensively utilized in COVID-19 research, vaccine development, therapeutic antibody screening, and diagnostic assay development, serving as a fundamental tool for understanding viral pathogenesis and developing countermeasures against coronavirus infections.
This product (SKU: RPCB1384) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 136.70 kDa with an observed molecular weight of 140-180 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant SARS-CoV-2 Spike S1+S2 ECD (S-ECD) (B.1.1.529/Omicron) Trimer Protein
SKU:
RPCB1384
Size:
100 μg
Reactivity:
SARS-CoV-2
Synonyms:
S protein, Spike glycoprotein, S glycoprotein, COVID-19
High quality, high purity and low endotoxin recombinant Recombinant SARS-CoV-2 Spike S1+S2 ECD(S-ECD)(B.1.1.529/Omicron) Trimer Protein (RPCB1384), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Recombinant SARS-CoV-2 Spike S1+S2 ECD(S-ECD)(B.1.1.529/Omicron) Trimer Protein was determined by Tris-Bis PAGE under reducing conditions.
Immobilized SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
