The RECQL4 Antibody (CAB6846) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the RecQL4 protein, it enables researchers to detect and analyze RecQL4 expression in a variety of cell types, making it particularly useful for studies in genetics, oncology, and aging research.RecQL4 is a member of the RecQ helicase family known for its role in maintaining genomic stability by resolving DNA damage and preventing mutations.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
RECQL4 Antibody
SKU:
CAB6846
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RECQ4, RECQL4
Positive Sample:
HeLa, Mouse thymus
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
133kDa
Observed MW:
140kDa
The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes.
Purification Method
Affinity purification
Gene ID
9401
RRID
AB_2767411
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RECQL4 Rabbit pAb (CAB6846) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
