The RGN Antibody (CAB3350) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Regucalcin, also referred to as senescence marker protein-30 (SMP30), is a multifunctional protein that plays a vital role in various physiological processes, including cell proliferation, apoptosis, and oxidative stress response. Its involvement in cellular signaling pathways makes it a valuable target for research in fields such as molecular biology, biochemistry, and aging studies.By utilizing the Regucalcin Polyclonal Antibody (CAB3350), researchers can effectively detect and analyze Regucalcin protein levels in different cell types, shedding light on its function in cellular processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RGN Antibody
SKU:
CAB3350
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RC, GNL, SMP30, HEL-S-41, RGN
Positive Sample:
Mouse liver, Rat kidney, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
33kDa
Observed MW:
35kDa
The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
9104
RRID
AB_2765067
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RGN Rabbit pAb (CAB3350) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
