The UPF1/RENT1 Monoclonal Antibody (CAB5071) is a high-quality antibody developed for reliable detection and analysis of target proteins. Rent1/Hupf1 is a key regulator of mRNA stability and has been linked to important cellular processes such as RNA degradation and gene expression.This monoclonal antibody, derived from rabbit hosts, has been extensively validated for use in Western blot applications, enabling researchers to reliably detect and analyze Rent1/Hupf1 protein levels in different cell types. Its high reactivity with human samples makes it an ideal choice for studies in molecular biology, RNA biology, and gene regulation.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UPF1/RENT1 Monoclonal Antibody
SKU:
CAB5071
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1268
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5976
RRID
AB_2863432
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using UPF1/RENT1 Rabbit mAb (CAB5071) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Confocal imaging of C6 cells using UPF1/RENT1 Rabbit mAb (CAB5071, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007,dilution 1:500)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
