The REV1 Polyclonal Antibody (CAB8493) is a valuable tool for researchers studying the REV1 protein, a key player in DNA repair mechanisms. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the REV1 protein, this antibody facilitates detection and analysis in various cell types, making it ideal for investigations in molecular biology and cancer research.REV1 is a crucial component of the DNA damage response pathway, playing a critical role in error-prone translesion synthesis during DNA replication.
Dysregulation of REV1 activity has been linked to genomic instability and tumorigenesis, making it a promising target for anticancer therapies. Research on REV1 may provide insights into mechanisms of mutagenesis and DNA repair, contributing to the development of novel treatments for cancer and other genetic disorders.
Product Name:
REV1 Rabbit Polyclonal Antibody
SKU:
CAB8493
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 120-345 of human REV1 (NP_057400.1).
This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA.
Purification Method:
Affinity purification
Gene ID:
51455
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of MCF-7 cells, using REV1 antibody (CAB8493) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
