The RFC4 Antibody (CAB5485) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It specifically targets the RFC4 protein, allowing for accurate detection and analysis in a variety of cell types.RFC4 plays a crucial role in maintaining genomic stability by assisting in the replication of DNA and ensuring proper repair of damaged DNA.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
RFC4 Antibody
SKU:
CAB5485
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
A1, RFC37, RFC4
Positive Sample:
HeLa, SW480, 22Rv1, SW620, K-562, HepG2, MCF7
Cellular Localization:
Nucleus.
Calculated MW:
40kDa
Observed MW:
40kDa
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Purification Method
Affinity purification
Gene ID
5984
RRID
AB_2766285
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RFC4 Rabbit pAb (CAB5485) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using RFC4 Rabbit pAb (CAB5485). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of K562 cells using 1 μg RFC4 antibody (CAB5485). Western blot was performed from the immunoprecipitate using RFC4 antibody (CAB5485) at a dilution of 1:1000.
