The RFK Antibody (CAB9141) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the RFK protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in biochemistry and molecular biology.RFK plays a crucial role in the synthesis of tetrahydrofolate, a key molecule in one-carbon metabolism essential for DNA synthesis and cell growth.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
RFK Antibody
SKU:
CAB9141
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RIFK, RFK
Positive Sample:
U-87MG, HeLa, 293T, Mouse small intestine, Mouse brain
Cellular Localization:
Cytoplasm.
Calculated MW:
18kDa
Observed MW:
14kDa
Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).
Purification Method
Affinity purification
Gene ID
55312
RRID
AB_2771988
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RFK Rabbit pAb (CAB9141) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Liu, Y., et al.
Targeting Riboflavin Kinase Induces Ferroptosis and Apoptosis in Triple-Negative Breast Cancer
