The RFWD2 Antibody (CAB10463) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to RFWD2, this antibody enables precise detection and analysis of the protein, making it an essential component of studies in genetics, cell biology, and cancer research.RFWD2, also known as E3 ubiquitin-protein ligase, plays a crucial role in maintaining genome integrity by regulating DNA repair pathways and cell cycle progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RFWD2 Antibody
SKU:
CAB10463
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FAP78, RFWD2, CFAP78, RNF200
Positive Sample:
HepG2, MCF7
Cellular Localization:
Cytoplasm, Nucleus Speckle.
Calculated MW:
80kDa
Observed MW:
110kDa
Enables ubiquitin protein ligase activity. Involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process; proteasome-mediated ubiquitin-dependent protein catabolic process; and response to ionizing radiation. Part of Cul4A-RING E3 ubiquitin ligase complex.
Purification Method
Affinity purification
Gene ID
64326
RRID
AB_2758012
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RFWD2 Rabbit pAb (CAB10463) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
