The RNASEH1 Antibody (CAB9116) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding specifically to Ribonuclease H1, this antibody allows for the detection and analysis of the enzyme in various cell types, making it ideal for investigations in molecular biology and RNA research.Ribonuclease H1 plays a crucial role in the processing of RNA molecules within cells, making it a key player in gene expression regulation. Its function in RNA degradation and RNA-DNA hybrid resolution has implications for diverse biological processes, including DNA replication, RNA maturation, and viral infection.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RNASEH1 Antibody
SKU:
CAB9116
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RNH1, H1RNA, PEOB2, RNASEH1
Positive Sample:
Mouse testis, Rat thymus
Cellular Localization:
Cytoplasm.
Calculated MW:
32kDa
Observed MW:
37kDa
This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17.
Purification Method
Affinity purification
Gene ID
246243
RRID
AB_2772028
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RNASEH1 Rabbit pAb (CAB9116) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
