The RNF144A Antibody (CAB17597) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and is validated for use in Western blot applications, making it a reliable choice for studies in immunology and cancer research.RNF144A, also known as E3 ubiquitin-protein ligase RNF144A, plays a crucial role in maintaining cellular homeostasis by targeting specific proteins for degradation through the ubiquitin-proteasome pathway. Its involvement in immune regulation suggests potential implications for diseases such as cancer, autoimmune disorders, and inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
RNF144A Antibody
SKU:
CAB17597
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UIP4, hUIP4, RNF144, UBCE7IP4, RNF144A
Positive Sample:
A-431, Rat brain
Cellular Localization:
Cytoplasm, Golgi Apparatus, Plasma Membrane.
Calculated MW:
33kDa
Observed MW:
38kDa
This gene encodes a member of a family of RING finger domain-containing E3 ubiquitin ligases that also includes parkin and parc. The expression of this gene is induced by DNA damage. The encoded protein interacts with the cytoplasmic DNA-dependent protein kinase, catalytic subunit (DNA-PKcs) and promotes its degradation through ubiquitination. The orthologous mouse protein has been shown to interact with a ubiquitin-conjugating enzyme involved in embryonic development.
Purification Method
Affinity purification
Gene ID
9781
RRID
AB_2772042
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RNF144A Rabbit pAb (CAB17597) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
