The RNF181 Antibody (CAB14405) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, and has been validated for use in Western blot applications.RNF181 is known to play a role in various cellular processes, including cell cycle regulation, DNA repair, and signal transduction pathways. Its dysregulation has been linked to diseases such as cancer, neurodegenerative disorders, and autoimmune diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RNF181 Antibody
SKU:
CAB14405
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HSPC238, RNF181
Positive Sample:
LO2, Mouse liver, Mouse brain, Mouse kidney, Mouse lung, Rat testis, Rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
18kDa
Observed MW:
18kDa
RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008 [PubMed 18331836]).
Purification Method
Affinity purification
Gene ID
51255
RRID
AB_2761274
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RNF181 Rabbit pAb (CAB14405) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
