The RNF220 Antibody (CAB15853) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the RNF220 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology, cell biology, and cancer research.RNF220, also known as a E3 ubiquitin-protein ligase, plays a pivotal role in protein degradation and signaling pathways within cells. Its functions in protein turnover and cellular signaling make it a promising target for research into various diseases, including cancer, neurodegenerative disorders, and metabolic conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RNF220 Antibody
SKU:
CAB15853
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HLD23, C1orf164, RNF220
Positive Sample:
HeLa, U-937
Cellular Localization:
Cytoplasm.
Calculated MW:
63kDa
Observed MW:
63kDa
Predicted to enable ubiquitin protein ligase activity. Involved in positive regulation of canonical Wnt signaling pathway. Part of protein-containing complex.
Purification Method
Affinity purification
Gene ID
55182
RRID
AB_2763280
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using RNF220 Rabbit pAb (CAB15853) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
