The RNF31 Antibody (CAB8227) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is optimized for use in Western blot applications. By binding specifically to the RNF31 protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it an ideal choice for studies in immunology and cancer research.RNF31 is a key player in the immune signaling pathway, particularly in the regulation of NF-kB activation and cytokine production.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
RNF31 Antibody
SKU:
CAB8227
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HOIP, Paul, ZIBRA, RNF31
Positive Sample:
Jurkat, MCF7, BT-474, HeLa, PC-3
Cellular Localization:
Cytoplasm.
Calculated MW:
120kDa
Observed MW:
125kDa
The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
55072
RRID
AB_2772048
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RNF31 Rabbit pAb (CAB8227) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
