The ROBO4 Antibody (CAB9876) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in various research applications, including Western blotting. By binding to the ROBO4 protein, this antibody enables precise detection and analysis in different cell types, making it an ideal choice for investigations in vascular biology and cardiovascular disease research.ROBO4, also known as Roundabout homolog 4, plays a crucial role in regulating blood vessel formation and stability, making it a promising target for studying angiogenesis and vascular abnormalities.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ROBO4 Antibody
SKU:
CAB9876
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MRB, AOVD3, ECSM4, ROBO4
Positive Sample:
HT-29, HepG2, HT-1080, HeLa
Cellular Localization:
Axon, Extracellular Exosome, Plasma Membrane.
Calculated MW:
107kDa
Observed MW:
130kDa
Predicted to enable cell-cell adhesion mediator activity. Involved in angiogenesis and establishment of endothelial barrier. Located in extracellular exosome. Implicated in aortic valve disease 3.
Purification Method
Affinity purification
Gene ID
54538
RRID
AB_2772057
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ROBO4 Rabbit pAb (CAB9876) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
