The RPN1 Polyclonal Antibody (CAB24537) is a high-quality antibody developed for reliable detection and analysis of target proteins. RPN1, also known as Ribophorin 1, is a protein involved in the process of glycoprotein folding and quality control in the endoplasmic reticulum.This polyclonal antibody, generated in rabbits, is highly specific to human RPN1 and is validated for use in various applications, including Western blotting and immunohistochemistry. By targeting the RPN1 protein, researchers can detect and analyze its expression in different cell types, shedding light on its function in protein folding and ER-associated degradation processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RPN1 Polyclonal Antibody
SKU:
CAB24537
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPN1, OST1, RBPH1, ribophorin I
Positive Sample:
SK-OV-3, Hep G2, BxPC-3, Mouse liver, Rat liver
Cellular Localization:
Endoplasmic Reticulum Membrane, Melanosome, Single-Pass Type I Membrane Protein.
Calculated MW:
68kDa
Observed MW:
68kDa
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
Purification Method
Affinity purification
Gene ID
6184
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using RPN1 Rabbit pAb (CAB24537) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
