The RPN1 Antibody (CAB6726) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the RPN1 protein, this antibody enables researchers to detect and analyze RPN1 expression in various cell types, making it ideal for investigations in areas such as cell biology and cancer research.RPN1, a subunit of the 26S proteasome, plays a crucial role in the recognition and degradation of misfolded or damaged proteins, thereby maintaining cellular homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
RPN1 Antibody
SKU:
CAB6726
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OST1, RBPH1, RPN1
Positive Sample:
HL-60, HepG2, SKOV3, MCF7, Rat liver
Cellular Localization:
Endoplasmic Reticulum Membrane, Melanosome, Single-Pass Type I Membrane Protein.
Calculated MW:
69kDa
Observed MW:
68kDa
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
Purification Method
Affinity purification
Gene ID
6184
RRID
AB_2767310
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RPN1 Rabbit pAb (CAB6726) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
