The RPP30 Antibody (CAB8479) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the RPP30 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and gene expression research.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RPP30 Antibody
SKU:
CAB8479
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TSG15, RPP30
Positive Sample:
HeLa, Jurkat, HT-1080, HepG2
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
29kDa
Observed MW:
29kDa
Enables ribonuclease P RNA binding activity. Contributes to ribonuclease P activity. Involved in tRNA 5'-leader removal. Part of multimeric ribonuclease P complex and ribonuclease MRP complex.
Purification Method
Affinity purification
Gene ID
10556
RRID
AB_2772090
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RPP30 Rabbit pAb (CAB8479) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using RPP30 Rabbit pAb (CAB8479) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
