The RPRD1A Antibody (CAB15854) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically targets RPRD1A and is highly reactive with human samples. Validated for use in Western blot applications, it enables the detection and analysis of RPRD1A in various cell types.RPRD1A is known to play a critical role in gene expression regulation, making it a key target for studies in molecular biology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RPRD1A Antibody
SKU:
CAB15854
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P15RS, HsT3101, RPRD1A
Positive Sample:
HT-29, HeLa, A-431, Mouse liver, Mouse testis, Rat brain
Cellular Localization:
Nucleoplasm, Nucleus, Rna Polymerase Ii, Holoenzyme.
Calculated MW:
36kDa
Observed MW:
36kDa
This gene encodes a cell-cycle and transcription regulatory protein. The encoded protein interacts with the cell cycle inhibitor cyclin-dependent kinase 4 inhibitor B and may function as a negative regulator of G(1)/S phase progression. This protein also forms homo- and hetrodimers with the protein, regulation of nuclear pre-mRNA domain-containing protein 1B, to form a scaffold that interacts with the C-terminal domain of RNA polymerase II subunit B1 and regulates several aspects of transcription. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 16.
Purification Method
Affinity purification
Gene ID
55197
RRID
AB_2763281
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RPRD1A Rabbit pAb (CAB15854) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
