The RPS6KA5 Monoclonal Antibody (CAB23891) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables ATP binding activity and protein serine/threonine kinase activity. Involved in several processes, including histone-serine phosphorylation; positive regulation of histone modification; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleoplasm.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Monkey samples.
Product Name:
RPS6KA5 Monoclonal Antibody
SKU:
CAB23891
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat, Monkey
Clone Number:
ARC61540
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MSK1, RLPK, MSPK1, RPS6KA5
Positive Sample:
SK-BR-3, C6, Mouse brain
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
62kDa/82kDa/90kDa
Observed MW:
80kDa/100kDa/80kDa/90kDa
Enables ATP binding activity and protein serine/threonine kinase activity. Involved in several processes, including histone-serine phosphorylation; positive regulation of histone modification; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleoplasm.
Purification Method
Affinity purification
Gene ID
9252
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using RPS6KA5 Rabbit mAb (CAB23891) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Mouse brain using RPS6KA5 Rabbit mAb (CAB23891) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
