Description
67kDa Laminin Receptor Antibody (CAB9008)
The 67kDa Laminin Receptor Antibody (CAB9008) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It targets the RPSA protein, allowing for the detection and analysis of RPSA expression in different cell types, making it an ideal tool for studies in cell biology and molecular biology research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | 67kDa Laminin Receptor Antibody |
| SKU: | CAB9008 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Sequence: | MSGA LDVL QMKE EDVL KFLA AGTH LGGT NLDF QMEQ YIYK RKSD GIYI INLK RTWE KLLL AARA IVAI ENPA DVSV ISSR NTGQ RAVL KFAA ATGA TPIA GRFT PGTF TNQI QAAF REPR LLVV TDPR ADHQ PLTE ASYV NLPT IALC NTDS PLRY VDIA IPCN NKGA HSVG LMWW MLAR EVLR MRGT ISRE HPWE VMPD LYFY RDPE EIEK EEQA AAEK AVTK EEFQ GEWT APAP EFTA TQPE VADW SEGV QVPS VPIQ QFPT EDWS AQPA TEDW SAAP TAQA TEWV GATT DWS | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | SA, LBP, LRP, p40, uS2, 67LR, ICAS, lamR, 37LRP, LAMBR, LAMR1, LRP/LR, LBP/p40, NEM/1CHD4, 67kDa Laminin Receptor |
| Positive Sample: | U-87MG, HeLa, B-cell, Mouse heart, Mouse kidney, Rat liver |
| Cellular Localization: | Cell Membrane, Cytoplasm, Nucleus. |
| Calculated MW: | 33kDa |
| Observed MW: | 37kDa/67kDa |
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
| Purification Method | Affinity purification |
| Gene ID | 3921 |
| RRID | AB_2772106 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit pAb (CAB9008) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
 | Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit pAb (CAB9008) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s. |
 | Immunofluorescence analysis of U-2 OS cells using 67kDa Laminin Receptor Rabbit pAb (CAB9008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
