The S100A8 Antibody (CAB15315) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific for human samples, making it ideal for use in Western blot experiments.S100A8, also known as calgranulin A, plays a critical role in various inflammatory processes and has been implicated in diseases such as cancer, inflammatory bowel disease, and rheumatoid arthritis. By binding to the S100A8 protein, this antibody enables researchers to detect and analyze its expression in different cell types, offering valuable insights into its function in both physiological and pathological conditions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
S100A8 Antibody
SKU:
CAB15315
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6279
RRID
AB_2762216
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using S100A8 Rabbit pAb (CAB15315) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using S100A8 Rabbit pAb (CAB15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human skin using S100A8 Rabbit pAb (CAB15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using S100A8 Rabbit pAb (CAB15315) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Confocal immunofluorescence analysis of U-2 OS cells using S100A8 Rabbit pAb (CAB15315) at dilution of 1:100. Blue: DAPI for nuclear staining.
