The SAP130 Antibody (CAB13897) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications. By targeting the SAP130 protein, researchers can effectively detect and analyze its expression in different cell types, providing insights into its role in biological pathways.SAP130 is a critical component of the splicing factor SF3b complex, which is involved in pre-mRNA splicing and spliceosome assembly. Dysregulation of SAP130 has been linked to various diseases, including cancer and neurodevelopmental disorders, highlighting its importance in cellular function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
SAP130 Antibody
SKU:
CAB13897
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SAP130
Positive Sample:
Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
110kDa
Observed MW:
150kDa
SAP130 is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex (Fleischer et al., 2003 [PubMed 12724404]).
Purification Method
Affinity purification
Gene ID
79595
RRID
AB_2760751
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse brain, using SAP130 Rabbit pAb (CAB13897) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
