The SCAP Antibody (PACO17051) is a highly specific and sensitive tool for researchers studying SCAP, a key regulator of cholesterol metabolism and homeostasis. This polyclonal antibody, produced in rabbits, is optimized for detecting SCAP in human samples and is suitable for use in Western blot applications. By targeting the SCAP protein, this antibody allows for precise identification and analysis of SCAP expression levels in various cell types, making it an essential tool for research in lipid metabolism and cardiovascular disease.SCAP, or SREBP cleavage-activating protein, plays a crucial role in the control of cholesterol biosynthesis and the regulation of lipid metabolism.
Dysregulation of SCAP has been linked to various diseases, including atherosclerosis and hyperlipidemia, making it a promising target for therapeutic intervention. The SCAP Antibody facilitates the investigation of SCAP function in these contexts, offering valuable insights into the mechanisms underlying lipid homeostasis and cardiovascular health. By understanding the role of SCAP, researchers can develop novel strategies for treating cardiovascular disorders and metabolic diseases.
Antibody Name:
SCAP Antibody (PACO17051)
Antibody SKU:
PACO17051
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fusion protein of human SCAP
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO17051(SCAP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO17051(SCAP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis.
Synonyms:
SREBF chaperone
UniProt Protein Function:
SCAP: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Belongs to the WD repeat SCAP family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 3p21.31Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; integral to membraneMolecular Function: protein binding; unfolded protein binding; cholesterol binding; protein complex bindingBiological Process: cholesterol metabolic process; regulation of fatty acid biosynthetic process; response to hypoxia; positive regulation of low-density lipoprotein receptor biosynthetic process; SREBP-mediated signaling pathway; negative regulation of cholesterol biosynthetic process; response to insulin stimulus; aging
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. [provided by RefSeq, Jul 2008]
sterol regulatory element-binding protein cleavage-activating protein
NCBI Synonym Full Names:
SREBF chaperone
NCBI Official Symbol:
SCAPÂ Â
NCBI Official Synonym Symbols:
NCBI Protein Information:
sterol regulatory element-binding protein cleavage-activating protein; SREBP cleavage-activating protein; sterol regulatory element binding protein cleavage-activating protein
UniProt Protein Name:
Sterol regulatory element-binding protein cleavage-activating protein
UniProt Synonym Protein Names:
Protein Family:
Sterol regulatory element-binding protein cleavage-activating protein
