The SCGB3A2 Antibody (CAB13137) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a secreted lung surfactant protein and a downstream target of thyroid transcription factor. A single nucleotide polymorphism in the promoter of this gene results in susceptibility to asthma. RRID AB_2759987 Gene ID 117156 Swiss Prot Synonym LU103; PNSP1; UGRP1; pnSP-1; SCGB3A2
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SCGB3A2 Antibody
SKU:
CAB13137
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LU103, PNSP1, UGRP1, pnSP-1, SCGB3A2
Positive Sample:
A-549, Raji, mouse lung, rat lung
Cellular Localization:
Secreted.
Calculated MW:
10kDa
Observed MW:
14kDa
The protein encoded by this gene is a secreted lung surfactant protein and a downstream target of thyroid transcription factor. A single nucleotide polymorphism in the promoter of this gene results in susceptibility to asthma. RRID AB_2759987 Gene ID 117156 Swiss Prot Synonym LU103; PNSP1; UGRP1; pnSP-1; SCGB3A2
Purification Method:
Affinity purification
Gene ID:
117156
RRID:
AB_2759987
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using SCGB3A2 Rabbit pAb (CAB13137) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunofluorescence analysis of C6 cells using SCGB3A2 Rabbit pAb (CAB13137) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using SCGB3A2 Rabbit pAb (CAB13137) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
