The SCN5A Antibody (CAB16665) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive to human samples, making it ideal for use in Western blot applications. By binding to the SCN5A protein, researchers can effectively detect and analyze its expression in various cell types, providing insights into cardiac function and potential implications for cardiovascular diseases.SCN5A, also known as the cardiac sodium channel, is crucial for the electrical signaling that regulates heart rhythm. Mutations in the SCN5A gene have been linked to various cardiac disorders, including long QT syndrome and Brugada syndrome.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
SCN5A Antibody
SKU:
CAB16665
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Endoplasmic Reticulum, Lateral Plasma Membrane, Nucleolus, Nucleoplasm, Perinuclear Region Of Cytoplasm, Plasma Membrane, Sarcolemma, T-Tubule, Z Disc.
Calculated MW:
227kDa
Observed MW:
230-280kDa
The protein encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. This protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene have been associated with long QT syndrome type 3 (LQT3), atrial fibrillation, cardiomyopathy, and Brugada syndrome 1, all autosomal dominant cardiac diseases. Alternative splicing results in several transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
6331
RRID
AB_2772163
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse lung, using SCN5A Rabbit pAb (CAB16665) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 40s.
