The CAVIN2 Polyclonal Antibody (CAB23889) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the Cavin2 protein, researchers can accurately detect and analyze its expression in a variety of cell types, making it an essential resource for studies in cell biology and signal transduction.Cavin2, also known as PTRF (polymerase I and transcript release factor), plays a crucial role in the organization and function of caveolae, specialized membrane structures involved in various cellular processes.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CAVIN2 Polyclonal Antibody
SKU:
CAB23889
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SDR, SDPR, PS-p68, cavin-2, CAVIN2
Positive Sample:
A-549, Mouse heart, Rat lung
Calculated MW:
47KDa
Observed MW:
47kDa
This gene encodes a calcium-independent phospholipid-binding protein whose expression increases in serum-starved cells. This protein is a substrate for protein kinase C (PKC) phosphorylation and recruits polymerase I and transcript release factor (PTRF) to caveolae. Removal of this protein causes caveolae loss and its over-expression results in caveolae deformation and membrane tubulation.
Purification Method
Affinity purification
Gene ID
8436
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CAVIN2 Rabbit pAb (CAB23889) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human spleen using CAVIN2 Rabbit pAb (CAB23889) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
