Description
The Do-It-Yourself SDS-PAGE Gel Kit from Assay Genie is a complete gel preparation system designed to simplify the casting of high-quality polyacrylamide gels for protein electrophoresis. This kit contains all essential reagents required for preparing both separating and stacking gels, enabling researchers to perform SDS-PAGE efficiently using standard electrophoresis equipment and deionized water.
Designed to streamline laboratory workflows and ensure reproducible gel polymerization, this system supports protein separation across a broad molecular weight range (6–15%). It is ideal for routine SDS-PAGE, protein expression analysis, purification workflows, and Western blot applications.
| Product Name: | Do-It-Yourself SDS-PAGE Gel Kit |
| SKU: | AGEL3522 |
| Size: | 10 Assays |
| Application: | SDS-PAGE Gel Preparation |
| Gel Range: | 6% – 15% Polyacrylamide |
SDS-PAGE (Sodium Dodecyl Sulphate–Polyacrylamide Gel Electrophoresis) is a widely used laboratory technique for separating proteins based on molecular weight. During electrophoresis, SDS denatures proteins and provides a uniform negative charge, allowing proteins to migrate through the polyacrylamide gel matrix according to size.
This kit provides optimized separating and stacking gel components to ensure clear band resolution and reproducible results across various molecular weight ranges, supporting downstream applications such as protein analysis and Western blotting.
| No | Component Name | 10 Assays | Storage |
| 1 | 30% Acr-Bis (29:1) | 45 mL | 2~8°C |
| 2 | Separating Gel Mix | 22 mL | Room Temperature |
| 3 | Stacking Gel Mix | 6 mL | Room Temperature |
| 4 | APS | 170 mg | Room Temperature |
| 5 | TEMED | 100 µL | 2~8°C |
| Form: | Liquid & Powder Components |
| Polymerization System: | APS/TEMED Initiated Polyacrylamide Polymerization |
| Storage Conditions: | Store components as indicated in Kit Components table |
| Recommended Use: | Prepare separating and stacking gels according to desired protein molecular weight range and experimental requirements. |
| Stability: | Stable for up to 6 months when stored properly |
