Description
The SDS-PAGE Tris-Glycine SDS Running Buffer (10X) from Assay Genie is a high-quality electrophoresis buffer formulated for use in sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE). Supplied as a convenient 10× concentrate, this buffer ensures efficient protein separation and reliable performance across routine laboratory workflows.
When diluted to 1× with deionized water, the buffer provides optimal ionic strength and pH conditions required for proper protein denaturation and migration during electrophoresis. It is suitable for use in standard vertical gel systems and downstream applications such as Western blotting.
| Product Name: | SDS-PAGE Tris-Glycine SDS Running Buffer (10X) |
| SKU: | AGEL3524 |
| Size: | 100 mL |
| Application: | SDS-PAGE Protein Electrophoresis |
| Concentration: | 10X Concentrate |
| Storage: | Room Temperature |
Tris-Glycine SDS running buffer is widely used in SDS-PAGE systems to maintain consistent pH and ionic strength during protein electrophoresis. The buffer system supports the denaturing conditions created by SDS, ensuring proteins migrate primarily according to molecular weight.
The Tris-Glycine system (25 mM Tris, 192 mM Glycine, 0.1% SDS at 1X concentration, pH 8.3) provides optimal conductivity and resolution for protein separation in polyacrylamide gels, making it suitable for routine laboratory applications and downstream protein analysis workflows.
| No | Component Name | Size | Storage |
| 1 | Electrophoresis Buffer (10X) | 100 mL | Room Temperature |
| Form: | Liquid |
| Working Concentration: | 1X (Dilute 1:10 with deionized water) |
| Composition (1X): | 25 mM Tris; 192 mM Glycine; 0.1% SDS; pH 8.3 |
| Preparation: | Combine 1 part 10X buffer with 9 parts deionized water and mix thoroughly |
| Stability: | Stable for up to 12 months when stored at room temperature |
| Precipitation Notice: | If precipitation occurs at low temperature, dissolve at 37°C before use |
