The SEC13 Antibody (CAB11613) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found.
This antibody is validated for use in WB, IF/ICC, ELISA, IHC applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SEC13 Antibody
SKU:
CAB11613
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISAIHC
Recommended Dilution:
WB
1:500 - 1:2000
IHC
1:50-1:200
IF/ICC
1:50-1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SEC13R, npp-20, SEC13L1, D3S1231E, SEC13
Positive Sample:
U-87MG, HeLa, 293T, HT-29, Mouse kidney, Mouse liver, Rat ovary
The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found.
Purification Method
Affinity purification
Gene ID
6396
RRID
AB_2758636
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SEC13 Rabbit pAb (CAB11613) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
