The SEL1L Antibody (CAB12073) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is part of a protein complex required for the retrotranslocation or dislocation of misfolded proteins from the endoplasmic reticulum lumen to the cytosol, where they are degraded by the proteasome in a ubiquitin-dependent manner. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2758977 Gene ID 6400 Swiss Prot Synonym Hrd3; SEL1L1; PRO1063; SEL1-LIKE; SEL1L
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SEL1L Antibody
SKU:
CAB12073
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Hrd3, SEL1L1, PRO1063, SEL1-LIKE, SEL1L
Positive Sample:
U-138MG, Mouse pancreas
Cellular Localization:
Endoplasmic Reticulum Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
89kDa
Observed MW:
100kDa
The protein encoded by this gene is part of a protein complex required for the retrotranslocation or dislocation of misfolded proteins from the endoplasmic reticulum lumen to the cytosol, where they are degraded by the proteasome in a ubiquitin-dependent manner. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2758977 Gene ID 6400 Swiss Prot Synonym Hrd3; SEL1L1; PRO1063; SEL1-LIKE; SEL1L
Purification Method:
Affinity purification
Gene ID:
6400
RRID:
AB_2758977
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using SEL1L Rabbit pAb (CAB12073) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of C6 cells using SEL1L Rabbit pAb (CAB12073) at dilution of 1:100. Blue: DAPI for nuclear staining.
