The SEPN1 Polyclonal Antibody (CAB15012) is a valuable tool for researchers studying SEPN1, a selenoprotein involved in cellular redox homeostasis and antioxidant defense mechanisms. This antibody, generated in rabbits, exhibits high specificity for SEPN1 in human samples and is suitable for use in Western blotting applications. By binding to the SEPN1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and oxidative stress research.SEPN1, also known as selenoprotein N, plays a crucial role in protecting cells from oxidative damage and regulating the cellular response to stress.
Its function in maintaining cellular health and preventing oxidative injury makes it a promising target for investigating conditions such as neurodegenerative diseases, muscle disorders, and aging-related pathologies. Understanding the role of SEPN1 is essential for developing strategies to enhance antioxidant defense mechanisms and mitigate the effects of oxidative stress in various disease states.
Product Name:
SEPN1 Rabbit Polyclonal Antibody
SKU:
CAB15012
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 341-590 of human SEPN1 (NP_065184.2).
This gene encodes a glycoprotein that is localized in the endoplasmic reticulum. It plays an important role in cell protection against oxidative stress, and in the regulation of redox-related calcium homeostasis. Mutations in this gene are associated with early onset muscle disorders, referred to as SEPN1-related myopathy. SEPN1-related myopathy consists of 4 autosomal recessive disorders, originally thought to be separate entities: rigid spine muscular dystrophy (RSMD1), the classical form of multiminicore disease, desmin related myopathy with Mallory-body like inclusions, and congenital fiber-type disproportion (CFTD). This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A second stop-codon redefinition element (SRE) adjacent to the UGA codon has been identified in this gene (PMID:15791204). SRE is a phylogenetically conserved stem-loop structure that stimulates readthrough at the UGA codon, and augments the Sec insertion efficiency by SECIS. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
57190
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SEPN1 Rabbit pAb (CAB15012) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5S.
