The SEPSECS Antibody (CAB7103) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the SEPSECS protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in biochemistry and molecular biology.SEPSECS plays a crucial role in selenocysteine biosynthesis, a unique amino acid crucial for the activity of selenoproteins involved in antioxidant defense, redox regulation, and thyroid hormone metabolism.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SEPSECS Antibody
SKU:
CAB7103
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LP, SLA, SecS, PCH2D, SLA/LP, SLA-p35, SEPSECS
Positive Sample:
293T, THP-1, Mouse kidney, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
56kDa
Observed MW:
56kDa
The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).
Purification Method
Affinity purification
Gene ID
51091
RRID
AB_2767658
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SEPSECS Rabbit pAb (CAB7103) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunofluorescence analysis of U2OS cells using SEPSECS Rabbit pAb (CAB7103). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
