The SERPINB9 Antibody (CAB6393) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for use in various experimental applications such as Western blotting.Serpin B9 is involved in inhibiting the activity of specific proteases, thereby playing a crucial role in controlling various physiological processes such as blood coagulation and inflammation. Dysregulation of Serpin B9 has been linked to several diseases, including cancer and chronic inflammatory conditions, making it a promising target for therapeutic intervention.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SERPINB9 Antibody
SKU:
CAB6393
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PI9, CAP3, PI-9, CAP-3, SERPINB9
Positive Sample:
SW480, SKOV3, Mouse lung, Mouse spleen, Rat spleen, Rat thymus
Cellular Localization:
Cytoplasm.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6.
Purification Method
Affinity purification
Gene ID
5272
RRID
AB_2766995
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SERPINB9 Rabbit pAb (CAB6393) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of C6 cells using SERPINB9 Rabbit pAb (CAB6393) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using SERPINB9 Rabbit pAb (CAB6393) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
