The SERPINB5 Antibody (CAB1179) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.Serpin B5, also known as Maspin, has been implicated in various physiological and pathological processes, including cancer progression, inflammation, and tissue remodeling.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SERPINB5 Antibody
SKU:
CAB1179
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PI5, maspin, B5
Positive Sample:
HeLa
Cellular Localization:
Secreted, Extracellular Space.
Calculated MW:
42kDa
Observed MW:
42kDa
Predicted to enable serine-type endopeptidase inhibitor activity. Predicted to be involved in negative regulation of endopeptidase activity. Predicted to act upstream of or within several processes, including extracellular matrix organization; prostate gland morphogenesis; and regulation of epithelial cell proliferation. Located in cytoplasm. Biomarker of hepatocellular carcinoma.
Purification Method
Affinity purification
Gene ID
5268
RRID
AB_2758757
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SERPINB5 Rabbit pAb (CAB1179) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa cells, using [KO Validated] SERPINB5 Rabbit pAb (CAB1179) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human stomach using SERPINB5 Rabbit pAb (CAB1179) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
