The PAI-1/Serpin E1 Antibody (CAB6211) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity and reactivity with human samples, making it a reliable choice for Western blot applications.Serpine1 is a key regulator of the fibrinolytic system, playing a crucial role in the inhibition of tissue plasminogen activators. Dysregulation of Serpine1 has been linked to various pathologies, including cardiovascular diseases, cancer metastasis, and fibrotic disorders.
This antibody is validated for use in WB, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PAI-1/Serpin E1 Antibody
SKU:
CAB6211
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PAI, PAI1, PAI-1, PLANH1, PAI-1/Serpin E1
Positive Sample:
Rat placenta
Cellular Localization:
Secreted.
Calculated MW:
45kDa
Observed MW:
48kDa
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. The protein also functions as a component of innate antiviral immunity. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.
Purification Method
Affinity purification
Gene ID
5054
RRID
AB_2766821
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat placenta, using PAI-1/Serpin E1 Rabbit pAb (CAB6211) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded human liver cancer using PAI-1/Serpin E1 Rabbit pAb (CAB6211) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
