The SETDB2 Antibody (CAB7391) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications. By targeting the SETDB2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for studies in epigenetics and cancer research.SETDB2 is a histone methyltransferase that plays a crucial role in gene silencing and chromatin modification, impacting various cellular processes such as DNA replication and repair.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
SETDB2 Antibody
SKU:
CAB7391
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLLD8, CLLL8, KMT1F, C13orf4, SETDB2
Positive Sample:
Mouse heart, Rat heart
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
82kDa
Observed MW:
100kDa
This gene encodes a member of a family of proteins that contain a methyl-CpG-binding domain (MBD) and a SET domain and function as histone methyltransferases. This protein is recruited to heterochromatin and plays a role in the regulation of chromosome segregation. This region is commonly deleted in chronic lymphocytic leukemia. Naturally-occuring readthrough transcription occurs from this gene to the downstream PHF11 (PHD finger protein 11) gene. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
83852
RRID
AB_2767923
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SETDB2 Rabbit pAb (CAB7391) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
