The SFRP2 Antibody (CAB5383) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the SFRP2 protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential component in studies related to developmental biology, tissue regeneration, and cancer research.SFRP2, also known as secreted frizzled-related protein 2, is a key player in regulating Wnt signaling pathways, which are crucial for normal development and control of cell proliferation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
SFRP2 Antibody
SKU:
CAB5383
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FRP-2, SARP1, SDF-5, SFRP2
Positive Sample:
Mouse kidney, Mouse heart
Cellular Localization:
Secreted.
Calculated MW:
33kDa
Observed MW:
35kDa
This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.
Purification Method
Affinity purification
Gene ID
6423
RRID
AB_2766193
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SFRP2 Rabbit pAb (CAB5383) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
