The SFRP4 Antibody (CAB6409) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is suitable for use in Western blot applications. By binding to SFRP4, researchers can detect and analyze this important protein in various cell types, making it an ideal choice for studies in developmental biology, cancer research, and regenerative medicine.SFRP4 is known to play a crucial role in regulating cell growth, differentiation, and tissue regeneration by modulating the Wnt signaling pathway.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SFRP4 Antibody
SKU:
CAB6409
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PYL, FRP-4, FRPHE, FRZB-2, sFRP-4, SFRP4
Positive Sample:
HepG2, 293T, HeLa, HO-8910
Cellular Localization:
Secreted.
Calculated MW:
40kDa
Observed MW:
40kDa/55kDa
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.
Purification Method
Affinity purification
Gene ID
6424
RRID
AB_2767011
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SFRP4 Rabbit pAb (CAB6409) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using SFRP4 Rabbit pAb (CAB6409). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
