The SFRP4 Antibody (CAB6409) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. RRID AB_2767011 Gene ID 6424 Swiss Prot Synonym PYL; FRP-4; FRPHE; FRZB-2; sFRP-4; SFRP4
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SFRP4 Antibody
SKU:
CAB6409
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PYL, FRP-4, FRPHE, FRZB-2, sFRP-4, SFRP4
Positive Sample:
HepG2, 293T, HeLa, HO-8910
Cellular Localization:
Secreted.
Calculated MW:
40kDa
Observed MW:
40kDa/55kDa
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. RRID AB_2767011 Gene ID 6424 Swiss Prot Synonym PYL; FRP-4; FRPHE; FRZB-2; sFRP-4; SFRP4
Purification Method:
Affinity purification
Gene ID:
6424
RRID:
AB_2767011
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SFRP4 Rabbit pAb (CAB6409) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using SFRP4 Rabbit pAb (CAB6409). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
