The SGK1 Antibody (CAB1025) is a high-quality antibody developed for reliable detection and analysis of target proteins. SGK1 is a serine/threonine protein kinase that plays a key role in cell survival, proliferation, and ion transport. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in Western blot applications.By binding specifically to SGK1, this antibody enables researchers to detect and analyze SGK1 expression in a variety of cell types.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
SGK1 Antibody
SKU:
CAB1025
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Purification Method
Affinity purification
Gene ID
6446
RRID
AB_2757776
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using SGK1 Rabbit pAb (CAB1025) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using SGK1 Rabbit pAb (CAB1025) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
