The SH3BGRL Antibody (CAB17530) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable SH3 domain binding activity. Located in extracellular exosome.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SH3BGRL Antibody
SKU:
CAB17530
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SH3BGR, HEL-S-115, SH3BGRL
Positive Sample:
Mouse lung, Mouse spleen, Mouse liver, Rat lung, Rat liver
Predicted to enable SH3 domain binding activity. Located in extracellular exosome.
Purification Method
Affinity purification
Gene ID
6451
RRID
AB_2772208
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SH3BGRL Rabbit pAb (CAB17530) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using SH3BGRL Rabbit pAb (CAB17530) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
