The SH3GL2 Antibody (CAB5701) is a high-quality antibody developed for reliable detection and analysis of target proteins. Endophilin A1 is a protein involved in membrane trafficking, specifically in endocytosis and synaptic vesicle recycling. This antibody, produced in rabbits, has high specificity and sensitivity for detecting Endophilin A1 in a variety of samples.With its validated use in Western blot applications, the Endophilin A1 Polyclonal Antibody enables accurate detection and analysis of Endophilin A1 protein levels in different cell types.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SH3GL2 Antibody
SKU:
CAB5701
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables identical protein binding activity. Involved in negative regulation of blood-brain barrier permeability; negative regulation of gene expression; and negative regulation of protein phosphorylation. Located in perinuclear region of cytoplasm.
Purification Method
Affinity purification
Gene ID
6456
RRID
AB_2766460
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SH3GL2 Rabbit pAb (CAB5701) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using SH3GL2 Rabbit pAb (CAB5701). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
