The Sheep Estrogen ELISA Kit is a reliable and accurate tool for detecting estrogen levels in sheep serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides precise and reproducible results, making it ideal for various research applications.Estrogen is a vital hormone in sheep physiology, playing a crucial role in reproductive function and overall health.
Monitoring estrogen levels is important for understanding sheep reproduction, managing breeding programs, and studying reproductive diseases.This ELISA kit is easy to use and offers a fast and efficient way to measure estrogen levels in sheep samples. Researchers and veterinarians can rely on this kit to support their studies on sheep reproduction, hormone regulation, and fertility management.
Product Name:
Sheep Estrogen ELISA Kit
Product Code:
SHFI00063
Size:
96 Assays
Detection method:
Competitive ELISA, Double Antibody
Application:
For quantitative detection of Estrogen in serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:
9.375pg/ml
Range:
15.625-1000pg/ml
Storage:
4°C for 6 months
Note:
For Research Use Only
Recovery:
Matrices spiked with levels of Estrogen. Recovery rates were calculated by comparing the measured value to the expected amount of Estrogen in samples. Please contact us for more information.
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estrogen and their serial dilutions. The results were demonstrated by percentage of calculated concentration to the expectation. Please contact us for more information.
CV(%):
Intra-Assay: CV<8% Inter-Assay: CV<10%
Component
Quantity
Storage
ELISA Microplate (Dismountable)
8×12 strips
4°C for 6 months
Lyophilized Standard
2
4°C/-20°C
Sample/Standard Dilution Buffer
20ml
4°C
Biotin-labeled Antibody(Concentrated)
120ul
4°C (Protect from light)
Antibody Dilution Buffer
10ml
4°C
HRP-Streptavidin Conjugate(SABC)
120ul
4°C (Protect from light)
SABC Dilution Buffer
10ml
4°C
TMB Substrate
10ml
4°C (Protect from light)
Stop Solution
10ml
4°C
Wash Buffer(25X)
30ml
4°C
Plate Sealer
5
-
Other materials and equipment required:
Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
Incubator
Deionized or distilled water
Absorbent paper
Buffer resevoir
Tsiartas et al.
Seven days ex vivo perfusion of whole ewe ovaries with follicular maturation and oocyte retrieval: towards the development of an alternative fertility preservation method
