The SHPRH Polyclonal Antibody (PAC017091) is a valuable tool for researchers studying the SHPRH protein, a key player in DNA repair processes. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the SHPRH protein, this antibody allows for accurate detection and analysis in various cell types, making it an essential component in studies related to DNA damage, genome stability, and cancer research.SHPRH, also known as E3 ubiquitin-protein ligase SHPRH, is essential for maintaining genomic integrity by mediating DNA repair and regulating cell cycle progression.
Dysregulation of SHPRH can lead to genome instability and increase susceptibility to genetic mutations, which are key factors in the development of cancer and other genetic disorders. The SHPRH Polyclonal Antibody provides researchers with a powerful tool to investigate the role of SHPRH in these processes and develop targeted therapies for diseases associated with DNA damage and repair abnormalities.
Antibody Name:
SHPRH Antibody (PACO17091)
Antibody SKU:
PACO17091
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:30-1:150
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human SHPRH
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO17091(SHPRH Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200).
Background:
SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).
Synonyms:
SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase
UniProt Protein Function:
SHPRH: E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. Belongs to the SNF2/RAD54 helicase family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.6.1.-; EC 3.6.4.-; EC 6.3.2.-; EC 6.3.2.19; Helicase; Ligase; Ubiquitin conjugating system; Ubiquitin ligaseChromosomal Location of Human Ortholog: 6q24.3Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activityBiological Process: protein polyubiquitination; response to DNA damage stimulus
UniProt Protein Details:
NCBI Summary:
SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).[supplied by OMIM, Mar 2008]
